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Stationary section chemistry dictates the affinity with the sample elements to stay or keep within the column because the cell period moves the sample throughout the column. Therefore, the sample elements traverse the column and elute at different rates.-hydroxybenzoic acid (PH) on a nonpolar C18 column matter to the highest analysis time of six mi
These brings about peak broadening, and that's why much better not to work with this kind of sovent. The choice of solvent depends upon the character of sample as well as sensitivity of the detector.Affinity chromatography: Affinity chromatography separates proteins based on their precise binding to a ligand immobilized on the stationary stage.It i
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